Dynamic Nanopore Microfluidic Transfection via Electroporation

Transfection, the process of delivering macromolecules such as DNA, mRNA, and proteins into cells, has enabled researchers to engineer cells for a variety of medical applications including cellular manipulation, cellular imaging, and genome engineering. Nanopore electroporation is a transfection tool that delivers macromolecules into cells through application of an electric field. When an electric field is applied, it is localized to the nanopores embedded in the insulated polycarbonate track etched (PCTE) membrane.

We have demonstrated successful delivery of Albumin from Bovine Serum (BSA), Alexa Fluor™ 488 conjugate into NIH 3t3 cells using nanopore electroporation.

Hoechst stain

BSA-Alexa Fluor 488


We will build upon this current technology of nanopore electroporation by incorporating flow in a microfluidic device.