Transfection, the process of delivering macromolecules such as DNA, mRNA, and proteins into cells, has enabled researchers to engineer cells for a variety of medical applications. Nanopore electroporation (NanoEP) is a gentle and highly efficient transfection method that utilizes an insulating nanoporous polycarbonate track-etched (PCTE) membrane to localize the electric field along the cell membrane over the nanopores. We are currently working on increasing the delivery efficiency and controllability of this transfection technique.
We have demonstrated successful delivery of Albumin from Bovine Serum (BSA), Alexa Fluor™ 488 conjugate into HT1080 cells using NanoEP.

Hoechst stain

BSA-Alexa Fluor 488
